Reporter

Part:BBa_I15003

Designed by: Jeff Tabor   Group: iGEM04_UTAustin   (2004-05-26)


3OC6HSL+LuxR dependent LacZalpha

System flanked on both sides by dual transcription terminators. Bears a joint Lux promoter-lambda operator, strong RBS, with LacZ alpha peptide. Requires LacZ omega complementation. See notes on E0033.

System dominantly repressed by lambda cI.


Usage and Biology

we're pretty sure E0033 is non-functional (JT 07.18.05)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 353
    Illegal XhoI site found at 404
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/reporter
Parameters
None